摘要
紫球藻 (Porphyridium cruentum)的藻胆蛋白粗提物经硫酸铵沉淀及透析后 ,分别用羟基磷灰石(HA)和 Sephadex G- 10 0两种方法柱层析 ,均可分离纯化出 B-藻红蛋白 (B- PE) ,纯度 (A54 5nm/ A2 80 nm)分别为 4 .92和 3.78。两种方法纯化后的 B- PE在聚丙烯酰胺梯度凝胶电泳时均得到一条带。B- PE的最大吸收峰在 545nm,其室温荧光发射峰为 574 .
The B Phycoerythrin(B PE) could both be separated after crude phycobiliproteins from Porphyridium cruentum were precipitated in ammonium sulphate and dialyzed,then chromatographed on a hydroxylapatite (HA) Column and a Sephadex G 100 column respectively.The purity of B PE separated by two methods was 4.92 and 3.78 (A 545 nm/A 280 nm) respectively.When the purified B PE was electrophoresed under polyacryiamide gradient gel electrophoresis condition,only one band was observed.The B PE possessed an absorption maximum at 545nm and a room fluoresence emission at 574.5nm.
出处
《中国海洋药物》
CAS
CSCD
2001年第3期33-35,24,共4页
Chinese Journal of Marine Drugs
关键词
紫球藻
B-藻红蛋白
分离
纯化
Porphyridium cruentum
B Phycoerythrin
purification
