螺旋藻不同组份对小鼠脾细胞分泌IL-2的促进作用

Study of synergistic effects of Spirulina platensis on inducing IL-2

本文采用 MTT法观察螺旋藻粗粉及提取物对 CTL L细胞和正常 BALB/ c小鼠脾细胞分泌 IL - 2的影响。实验结果表明 ,所用的螺旋藻的 3个组份在实验浓度范围内对 CTLL细胞无增殖活化作用、螺旋藻粗粉及提取物多糖、藻兰蛋白 1、0 .1和 0 .0 1g· L-1浓度时均对正常小鼠脾细胞分泌 IL - 2有促进作用 ,藻兰蛋白部分作用最强 ,其它提取物作用次之 ,在上述浓度范围内 IL - 2分泌水平有剂量依赖关系。高浓度 (1g· L-1)粗粉及提取物具有协同 Con A(2 mg· L-1)刺激小鼠脾细胞分泌 IL -

It has been shown that Spirulina platensis can regulate imminological functions.We report here that crude extract and purified components (phycocyanin and polysaccharide) from Spirulina platensis can induce secretion of IL 2 in splenocyte of BALB/C mice by means of MTT method.In the present study,we showed that all experimental components can't enhance proliferation of CTLL which was used in MTT method,but induce IL 2 secretion in splenocyte of BALB/C mice in three different concentration (0.01,0.1,1 g·L -1 ).Indeed the purified components especially phycocyanin part showed stronger IL 2 inducing activity than the crude one.IL 2 level was grow up when the incubation time of splenocyte and Spirulina platensis increased.In the concentration of 1 g·L -1 ,detected Spirulina platensis in our study assist IL 2 inducing of ConA (2mg·L -1 ) in splenocyte of BALB/C mice.