慢性冠状动脉狭窄及侧枝循环动物模型的建立

Animal model establishing in chronic myocardial ischemia and collateral circulation development

目的 :建立慢性冠状动脉狭窄及侧枝循环动物模型。方法 :健康小型猪 10只 ,开胸游离冠状动脉左回旋支(LCX)主干 ,采用改良Ameroid缩窄器 ,制作左回旋支慢性狭窄及缺血后侧枝循环生成模型。术前及术后 3周分别进行冠脉造影及超声心功能检查。结果 :4只LCX完全闭塞 ,5只狭窄大于 75 % ,心肌收缩及舒张功能明显下降。左前降支血管增粗、迂回 ,多支分支伸向缺血区形成血管网 ,无心肌梗塞表现。结论

Objective: To develop an animal model of chronic coronary stenosis and formation of collateral circulation in pigs.Method:Thoracotomy and pericardiectomy were performed in 10 pigs. Amerada constrictor was then implanted around the left circumflex artery proximal to the first marginal branch. Selective coronary angiography, Echo-Doppler study and pathological examination were performed pre and 3 weeks post surgery to evaluate the degree of stenosis, collaterals and myocardial function. Result:It was found that total occlusion in 4 and stenosis>75% in 5 pigs. Cardiac systolic and diastolic dysfunction were found in all subjects. Coronary collaterals formation were obvious.Conclusion:Ameroid constrictor is applicable to make an animal model of chronic coronary stenosis, thus stimulating collateral circulation formation, may create an important approach in research of cardiac rehabilitation.