维生素C对糖尿病大鼠尿白蛋白排泄率的影响

The effects of Vitamin C on urinary albumin excretion rate in rats with streptozotocin-induced diabetes

目的：研究不同剂量维生素C对糖尿病大鼠尿白蛋白排泄的影响。方法：实验大鼠随机分为五组：C组：正常对照；D组：糖尿病组；DT1组：糖尿病+维生素C30mg/kg/d；DT2组：糖尿病+维生素C90mg/kg/d；DT3组：糖尿病+维生素C 270mg/kg/d；分别在第1，2，4，6，8周测定尿白蛋白的排泄率，第9周测定血浆和肾脏维生素C水平。结果：与对照组相比，D组血浆、肾脏维生素C水平显著降低（Ｐ<0.05），DT1、DT2、和DT3组无显著性差异 。实验4、6、8周DT1、DT2、DT3组尿白蛋白的排泄率显著低于D组（Ｐ<0.05）。 DT1、DT2、DT3组相应各时相点之间尿白蛋白排泄率差异无显著性。结论：补充维生素C可显著降低糖尿病大鼠尿白蛋白的排泄率，维生素C可能对防治糖尿病肾病有一定作用。

Objective: To investigate the effects of the different doses of vitamin C on urinary albumin excretion rate （UAER）in diabetic rats. Methods: The rats were randomly divided into following groups: group C,control group; group D,streptozotocin-induced diabetic rats; group DT1,diabetic rats treated with vitamin C 30 mg/kg/d ; group DT2, diabetic rats treated with vitamin C 90; and group DT, diabetic rats treated with vitamin C 270 mg/kg/d. UAER were measured at 1,2,4,6 and 8 weeks, respectively. The concentrations of vitamin C in plasma and kidney were determined at 9 week. Results: Compared with group C, the levels of vitamin C in the plasma and kidney of group D decreased significantly（Ｐ<0.05）, which could be normalized by supplementation with vitamin C in group DT1, DT2 and DT3. At 4, 6 and 8 weeks, levels of UAER in the group DT1, DT2 and DT3 had markedly decreared compared with group D（Ｐ<0.05），and there were no significant difference among DT1, DT2 and DT3, respectively. Conclusion: UAER decreased significantly in diabetic rats that received vitamin C. Administration of vitamin C in diabetic patients may be beneficial in attenuating the progression of diabetic nephropathy.