应用ELISA法快速诊断伤寒副伤寒的研究

STUDY ON RAPIA DIAGNOSIS OF TYPHOID AND PARATYPHOIDFEVER.BY ELISA

应用自制伤寒、副伤寒杆菌可溶性抗原,包被聚苯乙烯微孔板,加入病人血清,使抗原抗体结合后,再加二抗酶结合物及TMBS 底物显色,以P/N≥2.1判为阳性,用于快速诊断伤寒结果较满意。全部实验仅2小时,用血10μl,检测伤寒标本(经肥达氏试验及细菌培养证实者)50例,阳性率为100%。其他疾病55例,正常人55例,皆无一例阳性反应。结果表明,该方法特异性强、灵敏度高、重复性好、并且具有快速、微量、简便、观察结果可避免人为误差等优点,易于基层推广应用。

Speedy diagnosis of typhoid with satisfactory results was carried out.Laboratory-made soluble antigen from typhoid bacilli was dripped into thetiny hoIes of a polystyrene plate and so was the patient's serum.When(?)the an ti-gen and antibody combined,an antienzyme combination and TMBS substrate wereadded,and then the mixture became coloured.It was positive if P/N(?)2.1,whichconfirmed the speedy diagnosis of typhoid.The whole test,took only 2 hours andonly 10 μl of blood was needed.55 typhoid samples(confirmed by Widal's test andbacterial culture) were tested and proved to be positive.55 non-typboid cases and55 heaithy persons were tested,but none turned out positive.It is suggested thatthis method is With strong specificity,high sensibivity and gratifying recurrence.And it can be conducted in a brief period of time,with a small amounl of blood,with a great deal of convenience,and without the occurrence of error due to humanfactors.It can be easily popularised in grass-roots units and rural areas.