污灌水对蚕豆根尖细胞及小鼠早期精细胞微核的影响

Effects of Sewage for Irrigation on Micronucleus Rates of Root-Tip Cells of Vicia Faba and Early Spermatids of Mice

目的 探讨灌溉用污水的细胞遗传毒性。方法 采集淮南市某蔬菜基地灌溉用污水沟内的水样进行蚕豆根尖细胞微核试验 ,并对水样有机提取物以腹腔注射进行小鼠早期精细胞微核试验。结果 按 1∶ 10、1∶ 2稀释的污灌水样及水样原液的蚕豆根尖细胞微核率均显著高于阴性对照 (P<0 .0 0 1) ,呈剂量 -反应关系 (r=0 .95 1,P<0 .0 5 ) ;污灌水样原液有机提取物小鼠早期精细胞微核率显著高于阴性对照 (P<0 .0 0 1)。结论 该地区灌溉用污水具有一定致突变作用 。

Objective To study the cytogenetic toxicity of sewage for irrigation Methods The sewage samples collected from a sewage ditch of a large scale vegetable farm were determined by micronucleus test of the root tip cells of Vicia faba in Huainan The organic extracts of the sewage samples were also determined by micronucleus test of early spermatids of mice exposed to organic extracts to be tested via peritoneal injection Results Significantly higher levels of micronucleus rates of the root tip cells of Vicia faba were observed in the original sewage samples and diluted sewage samples (1∶10,1∶2) compared with those of the negative control samples( P<0 001 ),and a significant dose response relationship( r=0 951,P<0 05 ) was developed The organic extracts of the original sewage samples revealed significantly higher micronucleus rates of early spermatids of mice compared with those of the negative control samples( P<0 001 ) Conclusion The sewage for irrigation presented mutagenicity to a certain degree and potential hazardous health effects on residents in sewage irrigation area