急性重症胆管炎大鼠肝肺组织内TNF_α、IL-1β的mRNA表达及活血清解灵的调节作用

Changes of mRNA Expression of IL-1β and TNF_α in Lung and Liver Tissues Acute Cholangitis of Severe Type (ACST)Rat Model and Regulation Effects of “Huo Xue Qing Jie Ling”

目的 :观察大鼠ACST时肝肺组织内TNFα、IL 1β的mRNA变化 ,以及应用活血清解灵进行中西医结合治疗的调节作用。 方法 :通过胆总管远端结扎 ,近端注入菌液并封闭的方法 ,造成大鼠ACST模型。采用单纯胆管减压、胆管减压配合活血清解灵两种不同方法治疗。应用原位杂交的方法检测肝肺组织内TNFα、IL 1β的mRNA表达。 结果 :IL 1β、TNFα 的mR NA杂交信号主要位于巨噬细胞内。模型组明显高于假手术组 ,中药组的阳性细胞数和表达量明显低于模型组 ,减压组与模型组相似。 结论 :ACST时肝肺巨噬细胞释放大量的炎性细胞因子 ,活血清解灵具有显著的免疫调节效应。

Objective:To observe the changes of the mRNA expression of IL 1 β and TNF α in the lung and liver tissues in acute cholangitis of severe type (ACST)model and to evaluate the regulation effects of “Huo Xue Qing Jie Ling” Methods:ACST rat models were made by distally ligating the choledochus with proximal injection of bacterial liquid.After either of the two therapies,including drainage of the common bile duct(CBD),drainage of CBD with oral injection of herbal decoction “Huo Xue Qing Jie Ling”,the mRNA expression of IL 1β and TNF α in the lung and liver tissues was observed by in situ hybridization.Results:The mRNA of IL 1β and TNF α in the lung and liver tissues is mainly located in the infiltrative macrophage.The expression in the model group was significantly higher than that in the pseudo operation control group.The positive cell number and expression in the drainage of CBD with herbal group were lower than those in the model group.The expression in the drainage of CBD group was similar to that in the model group.Conclusion:The macrophage of lung and liver in ACST released a large amount of IL 1β and TNF α.“Huo Xue Qing Jie Ling”can modulate the immune function.