两种定量测定丙型肝炎病毒核酸的聚合酶链反应方法比较

Two quantitative hepatitis C virus RNA reverse transcriptase PCR assays

目的 观察荧光定量聚合酶链反应 (PCR)PE5 70 0测定丙型肝炎病毒RNA的准确性。方法 所用临床评价血清盘由 36份血清组成 ,其中包括 1个 5倍倍比稀释系列 (7份 )、9份阴性血清和 2 0份阳性血清。使用临床评价血清盘比较 2种测定方法的重复性、线性和临床样本符合情况。结果 实时荧光定量方法和全自动PCR ELISA内标法检测系统的线性回归系数分别为 0 .9732和0 .9995。全自动PCR酶联免疫吸附 (ELISA)内标法检测系统不同含量的样本批内变异系数 (log10 )均比实时荧光定量方法低。临床样本的检测实时荧光定量方法与全自动PCR ELISA内标法检测系统的相关系数为 0 .845。结论 实时荧光定量方法具有良好的线性和重复性 ,同时与全自动PCR

Objective To compare the real time flourescent quantitative HCV RNA PCR detective method with automated quantitative PCR (COBAS Amplicor) test. Methods The test panel consisted of 36 samples including one half ten fold dilution series of 7 samples, 20 HCV RNA positive samples, and 9 negative samples. We compared the two quantitive method, with their agreement for test results, intra assay variability, and linearity. Results The regression coefficient of linearity of COBAS Amplicor and real time flourescent quantitative method was 0.999 5 and 0.973 2, respectively. The inter assay coefficient of variation of COBAS Amplicor was lower than that of the real time flourescent quantitative method. A correlation co efficient between the HCV RNA titres as detected by the two methods was 0.845 ( P <0.05). Conclusions Real time flourescent quantitative flourescent quantitative detection is reproducible and highly sensitive with a good correlation with COBAS Amplicor.