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两种定量测定丙型肝炎病毒核酸的聚合酶链反应方法比较 被引量:5

Two quantitative hepatitis C virus RNA reverse transcriptase PCR assays
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摘要 目的 观察荧光定量聚合酶链反应 (PCR)PE5 70 0测定丙型肝炎病毒RNA的准确性。方法 所用临床评价血清盘由 36份血清组成 ,其中包括 1个 5倍倍比稀释系列 (7份 )、9份阴性血清和 2 0份阳性血清。使用临床评价血清盘比较 2种测定方法的重复性、线性和临床样本符合情况。结果 实时荧光定量方法和全自动PCR ELISA内标法检测系统的线性回归系数分别为 0 .9732和0 .9995。全自动PCR酶联免疫吸附 (ELISA)内标法检测系统不同含量的样本批内变异系数 (log10 )均比实时荧光定量方法低。临床样本的检测实时荧光定量方法与全自动PCR ELISA内标法检测系统的相关系数为 0 .845。结论 实时荧光定量方法具有良好的线性和重复性 ,同时与全自动PCR Objective To compare the real time flourescent quantitative HCV RNA PCR detective method with automated quantitative PCR (COBAS Amplicor) test. Methods The test panel consisted of 36 samples including one half ten fold dilution series of 7 samples, 20 HCV RNA positive samples, and 9 negative samples. We compared the two quantitive method, with their agreement for test results, intra assay variability, and linearity. Results The regression coefficient of linearity of COBAS Amplicor and real time flourescent quantitative method was 0.999 5 and 0.973 2, respectively. The inter assay coefficient of variation of COBAS Amplicor was lower than that of the real time flourescent quantitative method. A correlation co efficient between the HCV RNA titres as detected by the two methods was 0.845 ( P <0.05). Conclusions Real time flourescent quantitative flourescent quantitative detection is reproducible and highly sensitive with a good correlation with COBAS Amplicor.
出处 《中华检验医学杂志》 CAS CSCD 北大核心 2001年第3期146-147,共2页 Chinese Journal of Laboratory Medicine
关键词 C型肝炎样病毒属 丙型肝炎 HCV 聚合酶链反应 病毒RNA Hepatitis C like viruses Polymerase chain reaction Virus RNA
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参考文献1

  • 1Didomenico N,Clin Chem,1996年,42卷,1915页

同被引文献56

  • 1曹文俊,拉夏德,吴华成,顾志冬,朱正纲,樊绮诗,王亚新,许叔祥.逆转录-实时定量PCR检测胃癌和肠癌病人CEA基因的表达[J].诊断学理论与实践,2002,1(1):22-24. 被引量:6
  • 2Gallarda JL, Dragon E. Blood screening by nucleic acid amplification technology: current issues, future challenges [J].Mol Diag,2000;5:11-22.
  • 3Busch MP,Kleinman SH,Jackson B,et al. Nucleic acid amplification testing of blood donors for transfusion-transmitted infectious diseases [J]. Transfusion, 2000 ; 40 : 143.
  • 4Heid CA, Stevens J, Livak KJ,et al. Real time quantitative PCR[J]. Genome Res, 1996 , 6 : 986-994.
  • 5Cuijpers HTM,Molijn MHJ, Bos HJ,et al. Validation of the Nuclei Sens Extractor in combination with the hepatitis C virus Cobas Amplicor 2.0 assay in four laboratories technology for blood screening[J]. Vox Sang, 2001 ; 81 : 12 - 20.
  • 6Cuijpers HTM,Molijn MHJ,Bos HJ,et al. Validation of the Nuclei Sens Extractor in combination with the hepatitis C virus Cobas Amplicor 2.0 assay in four laboratories technology for blood screening[J]. Vox Sang,2001 ;81 :12~20.
  • 7Gallarda JL, Dragon E. Blood screening by nucleic acid amplification technology : current issues, future challenges [J].Mol Diag,2000; 5 : 11-22.
  • 8Busch MP,Kleinman SH,Jackson B,et al. Nucleic acid amplification testing of blood donors for transfusion-transmitted infectious diseases[J]. Transfusion, 2000 ; 40:143.
  • 9Heid CA, Stevens J, Livak KJ,et al. Real time quantitative PCR[J]. Genome Res, 1996;6 : 986- 994.
  • 10Wend LW,Mel K,John C,et al.Evaluation of roche amplicor PCR assay for mycobacterium tuberculosis[J].Jclin Microbiol,1996,34:134-139.

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