P27、P53和增殖细胞核抗原在胃癌组织中的表达及其临床意义

Analysis of relationship between P27, P53 and PCNA expression and its clinical significance

目的 :探讨 P2 7、P5 3和增殖细胞核抗原 (PCNA)在胃癌组织中的表达及其与临床病理指标及预后的相关性。方法 :应用免疫组织化学 SP法 ,检测 6 2例人胃癌组织中 P2 7、P5 3、PCNA的表达。结果 :胃癌组织中 ,P2 7、P5 3、PCNA表达的阳性率分别为 37.1%、40 .4%、83.9%。 P2 7的表达与 Bormann分型、肿瘤浸润深度、淋巴结及远处转移、临床分期有关 ;P5 3表达与患者性别、远处转移、临床分期有关 ;而 PCNA仅与患者年龄、肿瘤分化程度有关。P2 7阳性组 5年生存率高于阴性组 ,P2 7与 PCNA表达呈显著负相关。 结论 :胃癌组织中 P2 7、P5 3、PCNA的表达可作为判断肿瘤恶性程度、有无转移及预后的重要参考指标。

Objective: To investigate the relationship between P27,P53 and PCNA expression in human gastric carcinoma tissues and clinicopathological parameters. Methods: The expression of P27,P53 and PCNA in 62 human gastric carcinoma tissues was examined with immunohistochemistry SP method. Results: Positive rates of P27,P53 and PCNA expression were 37.1%, 40.4%,83.9%. P27 expression was related with Bormann type, infiltrative depth, lymph node and distant metastasis and clinical stage. P53 expression was related with sex of patients, distant metastasis and clinical stage. PCNA expression was related with age of patients and infiltrative depth of tumor. P27 positive expression group was higher than negative group as to 5 year survival. P27 expression was in reverse relation with PCNA expression. Conclusion: The expression of P27, P53 and PCNA may be regarded as an important marker in judging malignant degree of gastric carcinoma,distant metastasis and prognosis.