胰岛素样生长因子Ⅰ对体外培养人牙周膜细胞骨钙素分泌的影响

Effect of insulin-like growth factor-Ⅰ on osteocalcin secretion of periodontal ligament cells

目的 :观察胰岛素样生长因子 (IGF- )对体外培养的人牙周膜细胞骨钙素分泌的影响 ,以探讨 IGF- 在人牙周膜细胞向成骨样细胞分化中的作用。 方法 :体外培养牙周膜细胞 ,放免法测定人牙周膜细胞骨钙素分泌量。实验分为空白对照组、IGF- 3.12 5 ng/ ml组、IGF- 6 .2 5 0 ng/ ml组、IGF- 12 .5 0 0 ng/ ml组、IGF- 2 5 .0 0 0 ng/ m l组。结果 :在含 β-甘油磷酸钠和 L -抗坏血酸的条件下 ,人牙周膜细胞体外培养可产生并分泌骨钙素 ;随着培养时间的延长 ,骨钙素分泌增加 ,在第 3周时分泌量达最大。加入生长因子 IGF- ,骨钙素分泌量呈剂量依赖性增加。结论 :IGF- 可增加人牙周膜细胞骨钙素的分泌。

Objective: To investigate the effect of insulin like growth factor Ⅰon osteocalcin secretion in periodontal ligament cells. Methods: Human periodontal ligament cells were cultured by tissue explant in vitro , and the concentration of osteocalcin were determined with radio immunological method. Results: Under the condition of L ascorbic acid and β glycerophosphate in culture medium, human periodontal ligament cells secreted osteocalcin time dependently and peaked at the third week; IGF Ⅰ3.125 ng/ml,6.250 ng/ml, 12.500 ng/ml, 25.000 ng/ml could promote the secretion of osteocalcin dose dependently. Conclusion: IGF Ⅰ can increase the secretion of osteocalcin in human periodontal ligament cells.