新生鼠呼吸道感染CVB_3致气道炎症的病理学观察

Pathological observation of airway inflammation after neonatal CVB_3 inoculation in rats

目的 :观察病毒感染对气道炎症、肺形态学的影响以及持续的气道反应性增高 ,探讨病毒感染与哮喘的关系。方法 :建立新生大鼠病毒感染模型 ,出生 5 d新生鼠超声雾化吸入柯萨奇病毒 B3(CVB3) ,接种后 10 d取血查 CVB3- Ig M;接种后 10d、30 d分别观察湿肺质量与鼠体质量比值 (L W/ BW)、气道肺病理学改变。 结果 :病毒组新生鼠血清中 CVB3- Ig M的 D值均在对照组 x+2 s以上接种后 10 d L W/ BW比值病毒组明显高于对照组 (P<0 .0 1)。病理学检查 :急性期炎症改变显著 ;接种后30 d仍有持续的形态学及细胞学改变。 结论 :新生鼠吸入 CVB3模型成立 ,并有持续的气道炎症及肺形态学改变。

Objective: To observe the effect of coxsackie virus B 3 on airway tract and lung morphology, and to study the relation between CVB infection and asthma. Methods: We established CVB 3 infective model: 5 d neonatal rats inhaled CVB 3 by ultrasonic brume. CVB 3 IgM was examined 10 d after inoculating of CVB 3, and LW/BW, airway tract and lung pathological change 10 d and 30 d after inoculation of CVB 3 were observed. Results: Rats from the virus group had higher D of CVB 3 IgM than control's ( +2s ) and had higher LW/BW 10 d after inoculation of CVB 3 than control ( P <0.01). Neonatal rats had acute inflammatory changes 10 d after inoculation of CVB 3 and persistent changes in morphology and cytology. Conclusion: Neonatal rats virus model is established. Respiratory infection by CVB 3 in neonatal rats has persistent changes in airway tract inflammatory and morphology.