小鼠卵巢组织的体外培养及形态学观察

Study on The Histologic Change of Rat Ovary Tissue After Culturing in Vitro

目的 :探讨提高移植物成活率的最佳培养时间和培养条件。方法 :将小鼠卵巢在体外培养 1d、2d、3d后 ,分别观察培养前后卵巢组织的酶活性及形态学变化。结果 :在合理的体外条件下 ,小鼠卵巢内的卵泡有继续发育的趋势 ;酶活性显示 ,培养 2d之内的酶活性与正常的相比无明显差异。结论 :在同等培养条件下 ,卵巢培养最佳时间为

Objective:To search for the most adequate duration and conditions of tissue culture to reduce the immunogenicity and therefore raise survival rate of the cultivated tissue after transplantation.Methods:The changes of morphology and enzymatic activity of the cultivated ovary tissue were observed before and after cultivating for 1d、2d and 3d respectively.Results:Ovarian enzyme activity appeared significant differences as compared with unculfured ovary within two day's culture.Conclusion:Under the same cultural conditions,the most appropriate cultural duration for the rat's ovary tissue is within two days.