产超广谱β-内酰胺酶(ESBL)细菌的药敏检测分析

Antimicrobial susceptibility of resistant bacteria with extended-spectrum β-lactamase(ESBL)

目的:通过对１２９株产超广谱β－内酰胺酶(ＥＳＢＬ)细菌的分析,对临床针对性治疗ＥＳＢＬ耐药菌提出建议。方法:应用双纸片法测定临床分离的可疑产ＥＳＢＬ的大肠埃希氏菌和肺炎克雷伯氏菌。结果:２１０株大肠埃希氏菌ＥＳＢＬ阳性检出率为３６%,７７株肺炎克雷伯氏菌ＥＳＢＬ阳性检出率为６８%。分离的１２９株ＥＳＢＬ细菌对青霉素类,一代、二代和三代头孢菌素。氨曲南耐药,对喹诺酮类、氨基糖苷类、复方磺胺耐药率在５０%以上。结论:ＥＳＢＬ细菌的监测对指导临床用药具有重要意义。

Objective: One hundred and twenty - nine strains of resistant bacteria with extended - spectrum β - lactamase (ESBL) were analied in order to provide the suggestions of empirical treatment against the kind of bacteria. Methods: Identification of bacteria with ESBL including E. coli and KL. pneumonia were performed by using double -disk diffusion tests. Results: Of 210 strains of E. coli, 36% were with ESBL, while KL. pneumonia with ESBL accounted for 68% in all isolats of KL. pneumonia. In 129 strain bacteria with ESBL, All were risisant to penicillins, the first, the second and the third generation of cephalosporins and aztreonan, while more than 50% were risistant to guinolones, aminoglycosides and sulfamethoxazole/trimethoprin. Conclusion: Monitoring the ESBL bacteria is important for direction of clinical using drugs.