慢性肝病患者PBMC中TGF-β_1mRNA表达及其与肝纤维化的关系

Correlation of TGF-β_1 mRNA expression in peripheral blood mononuclear cells with liver fibrosis in chronic liver disease

目的 :研究转化生长因子 β1(transforminggrowthfactor β1,TGF β1)在慢性肝病患者外周血单个核细胞 (peripheralbloodmononuclearcell,PBMC)中的表达及其与肝纤维化的关系。方法 :用定量RT PCR法 (RT PCR加Dotblot法 )检测轻、中、重度慢性肝炎、肝炎后肝硬变活动期患者和与之对照的正常人PBMC中TGF β1mR NA水平 ,并对其与肝纤维化血清学指标的关系进行统计学分析。结果 :慢性轻、中、重度肝炎和肝炎后肝硬变活动期PBMC中TGF β1mRNA分别为 1.47± 0 .47、1.90± 0 .5 3、3.0 9± 1.44和 3.2 1± 0 .91,显著高于对照组(0 .78± 0 .41) (P <0 .0 1)。肝纤维化血清学指标增高≥三项患者组和增高 <三项患者组PBMC中TGF β1mR NA分别为 2 .6 2± 1.14和 1.6 1± 0 .5 1,亦显著高于对照组 (P <0 .0 1,P <0 .0 5 ) ,其中增高≥三项患者组的TGF β1mRNA水平尚高于增高 <三项患者组 (P <0 .0 1)。结论 :TGF β1在慢性病毒性肝炎患者PBMC中表达增高 。

Objective:To study the TGF β 1 mRNA expressions in peripheral blood mononuclear cell (PBMC) of chronic liver disease and their correlation with liver fibrosis.Methods:TGF β 1 mRNA expressions were analyzed in PBMC from patients with mild chronic hepatitis, intermediate chronic hepatitis, severe chronic hepatitis, active liver cirrhosis using a quantitative reverse transcriptase polymerase chain reaction technique. Moreover, the serum contents of collagen type Ⅳ(C Ⅳ), procollagen Ⅲ(PC Ⅲ), laminine(LN) and hyaluronic acid(HA) were determined using radioimmunoassay.Results:The patients with chronic liver disease had significantly higher PBMC TGF β 1 mRNA levels (1.47±0.47, 1.90 ± 0.53,3.09±1.44 and 3.21±0.91) respectively than those in normal subjects(0.78±0.41)(P<0.001). All the patients were divided into two groups—group with at least three abnormal items from the four serum markers of liver fibrosis(C Ⅳ,PC Ⅲ,LN and HA) and group with less than three abnormal items. Both groups had significantly higher PBMC TGF β 1 mRNA levels(2.62±1.14 and 1.61±0.51) respectively than those in normal subjects(P<0.001). Furthermore, the group with at least three abnormal items had significantly higher PBMC TGF β 1 mRNA levels than those in the group with less than three abnormal items(P<0.001).Conclusion:PBMC TGF β 1 mRNA levels increase remarkably in patients with chronic viral hepatitis and are associated with the activity of liver fibrosis.