摘要
目的 观察碱性成纤维细胞生长因子 (basic fibroblast growth factor,b FGF)对兔晶状体上皮细胞 (rabbit lens epithelial cells,RL ECs)的作用。方法 采用 b FGF作用于第 2~ 3代培养细胞 ,用 MTT法测定对细胞增殖的影响 ;电镜观察细胞形态的变化 ,流式细胞仪观察细胞周期的变化。结果 b FGF可促进晶状体上皮细胞的增殖 ,尤其是浓度为10 μg· L- 1 作用最明显。透射电镜显示细胞增殖活跃 ,流式细胞仪观察进入 S期的细胞明显增加。结论 b FGF是促进培养兔晶状体上皮细胞增殖的重要因素。
Objective To study the effect of basic fibroblast growth factor (bFGF) on proliferating of rabbit lens epithelial cells(RLECs).Methods The second and three generations of RLECs were exposed to different concentrations of bFGF, and the proliferation characteristics of the cells were measured with methyl thiazolyl tetrazolium (MTT) assay. The ultrastructure of cell were observed with transmission electron microscope (TEM).The changs of cells cycles were observed with flow cytometer (FCM).Results After the treatment of bFGF, RLECs showed the marked proliferation , especially in 10μg·L -1 of bFGF . TEM result showed that the cells were more active with bFGF, FCM result showed that the S phase cell obviously increased.Conclusion bFGF is an important factor that can promote proliferation of RLECs.
出处
《眼科新进展》
CAS
2001年第3期161-163,共3页
Recent Advances in Ophthalmology