Leflunomide抑制大鼠角膜移植免疫排斥反应的研究

Effects of immunosuppressant Leflunomide on penetrating keratoplasty rejection model in rat

目的 研究 L eflunomide对大鼠角膜移植排斥反应的防治作用。方法 建立大鼠穿透性角膜移植排斥反应的动物模型 ,观察 L eflunomide对大鼠角膜植片存活和排斥反应指数 (RI)的影响 ,并与阴性对照组和 Cs A治疗组相比较。结果 阴性对照组角膜植片存活时间为 12 .375 d± 1.76 8d,而 Cs A组为 17.375 d± 1.40 8d,L eflunom ide组为 18.2 5 0 d±1.35 6 d,均比阴性对照组显著延长 (P<0 .0 1)。结论 L eflunomide能抑制大鼠穿透性角膜移植免疫排斥反应 ,显著延长角膜植片的存活时间。

Objective To study the efficacy of immunosuppressant Leflunomide on the rat model of penetrating keratoplasty.Methods Corneal grafts were transplanted orthotopically from Lou rats to F344 rats recipients. Animals were randomly assigned to the following groups: (1) control; (2) CsA (5mg·kg -1 ·d -1 , po ); (3) Leflunomide (5mg·kg -1 ·d -1 , po ). Treatment was begun on the first postoperative day and continued until the 14th day . Mean survival times and rejection index were determined.Results The mean survival time of the control group was merely 12.375 d±1.768d, But the mean survival time of the treated groups with CsA and Leflunomide were respectively 17.375 d ±1.408 d and 18.250 d±1 356 d,which was statistically prolonged compared with the control group( P <0.01).Conclusion Leflunomide can markedly prolongs the survival time of corneal allografts by inhibiting graft rejection.