腹膜毛细淋巴管网的形态结构特征

MORPHOLOGICAL AND STRUCTURAL CHARACTERISTICS OF THE PERITONEAL LYMPHATIC CAPILLARY NETWORKS

目的 探讨腹膜淋巴管的形态特征 ,为研究内皮和间皮细胞的物质转运机理提供必要的依据。 方法 用酶组织化学染色法观察了猴腹膜毛细淋巴管网的分布及其内皮细胞的超微结构。 结果 腹膜毛细淋巴管有丰富的网状连接、显著的瓣样结构和大量盲端 ,管径变化较大 (约 40～ 12 0 μm)、管腔极不规则。淋巴管周围结缔组织稀疏 ,并常见到内皮细胞与覆盖其表面的间皮细胞直接相贴。网膜和系膜内可见由大量巨噬细胞及淋巴细胞构成的圆形或椭圆形的乳斑 ,起于乳斑的淋巴管与间皮细胞之间缺乏基底膜。膈腹膜小孔的边缘附着有大量的细纤维成分 ,形成小孔的间皮细胞与其深面的淋巴管窦均有较强的 5’ Nase酶活性。 结论 腹膜毛细淋巴管的形态及结构显示出明显的部位性差异 。

Objective Peritoneal lymphatic features are studied to provide necessary data for understanding transport capabilities of the endothelial and mesothelial cells. Methods Enzyme\|histochemical staining methods were employed to investigate organization of the lymphatic networks and their endothelial ultrastructures in the monkey peritoneum using light,scanning and transmission electron microscopy. Results 5' Nase positive initial lymphatics showed extensive network,obvious valve like structures and numerous blind ends.The calibre of lymphatics with extremely irregular lumen varied greatly from 40 to 120μm.Lymphatic endothelium was usually separated from mesothelium by a small quantity of loose connective tissue,or they directly contacted each other in some areas.No basal lamina occurred between the peritoneum and the lymphatics originating from milky spots in the omentum and mesovarium.Milky spots are oval or round visible bodies aggregated by macrophages and lymphocytes.Both the endothelium of lymphatic lacunae and the mesothelium that forms the peritoneal stomata,represent a strong 5' Nase activity.Abundant microfibrils attached the stomatal edge. Conclusion The distribution and structure of peritoneal initial lymphatics reveal significant regional variations,and lymphatic endothelium has a close morphological and functional relationship with the mesothelium. [