mRNA差异显示法克隆小鼠精子发生相关基因-p38MAPK基因

CLONING OF SPERMATOGENESIS RELATED GENE p38 MAPK GENE FROM TESTIS TISSUE OF MOUSE

目的 克隆 1、2、3、4周龄小鼠睾丸之间差异表达的基因。 方法 应用改良的mRNA差异显示技术 ,对 1、2、3、4周龄小鼠睾丸进行了基因表达的差异显示分析 ,并对其中 1个从 2周龄小鼠中高表达的cDNA片段进行了克隆和测序。 结果 所克隆的cDNA片段与小鼠大脑、造血干细胞p38MAPK(p38betamitogen activatedpro teinkinase ,p38MAPK β)基因的同源性分别为 91%和 85 %。Northerndotblot结果显示该基因在小鼠睾丸和脑组织中表达最高。

Objective To clone some genes different expressed among 1,2,3 and 4 week old mouse. Methods The differences of gene expression among 1,2,3 and 4 week old mouse were analyzed with improved mRNA differential display techniques. Results Significant different expressed gene was observed among the four tissues, and a sequence tag highly expressed in 2 week old mouse was cloned and sequenced. Homology analysis indicated that the EST shared 91% and 85% homology with mouse brain and hematopoietic stem cell p38 MAPK. Northern dot blot identified the expression in testis and brain is the highest. Conclusion These data suggested that the p38 MAPK gene might be associated with spermatogenesis. [