小鼠肾脏发育中的细胞凋亡

APOPTOSIS IN THE DEVELOPMENT OF KIDNEY IN MOUSE

目的 研究小鼠肾脏发育过程中的细胞凋亡规律及形态学特点。 方法 应用光镜、电镜技术和TUNEL法分别对不同胚龄、生后日龄小鼠肾脏细胞凋亡进行了观察。 结果 皮质凋亡细胞多出现在生肾区S小体之间和肾小体内 ,凋亡高峰期在胚龄 14～ 18d之间。髓质凋亡细胞出现在肾小管上皮内 ,凋亡高峰期在生后 7d左右。超微结构观察可见皮质和髓质凋亡细胞主要表现为核固缩 ,染色质凝集 ,细胞皱缩。皮质和髓质凋亡细胞结局为 ,被邻近细胞吞噬 ,或脱落到肾小管腔内。 结论 小鼠肾脏发育过程中确有细胞凋亡 ;皮质中细胞凋亡与生肾区的出现和肾小体发育完善有关 。

Objective To observe apoptosis of renal development in mouse. Methods Light,electron microscopy and TUNEL method were used. Results The apoptosis could be easily found in cells between S like body in nephrogenic zone and in renal corpuscles before birth, especially at 14 18 days prenatally. The apoptotic cells in epithelia of renal tubule or collecting duct could be observed in postnatal renal medulla. In medullary papilla the cell death peaked around postnatal day 7. Electron microscopy revealed that apoptotic cells were ingested by neighboring cells. Some apoptotic cells detached from epithelia into the lumen of tubule or duct. Conclusion This study provides morphologically some evidences of apoptosis in the mouse kidney embryonically and postnatally. The apoptosis in cortex was apparently related to the development of nephrogenic zone and renal corpuscles; and the apoptosis in medulla might play an important role in the development of renal tubular epithelia. [