蝌蚪提取液对HL-60细胞相关癌基因表达的影响

EFFECTS OF TADPOLE EXTRACT ON EXPRESSION OF THE RELATED ONCOGENES IN HL 60 CELLS

目的 研究蝌蚪提取液 (T871 3)对白血病细胞的作用机制。 方法 利用细胞形态学观察、细胞化学、TUNEL法等技术 ,并采用原位mRNA杂交和完整细胞原位斑点印迹技术 ,观察T871 3诱导HL 6 0细胞分化和凋亡过程中c myc、c myb、bcl 2癌基因表达的变化。 结果 1 T871 3能抑制HL 6 0细胞分裂增殖。 2 T871 3在低浓度时诱导HL 6 0细胞向单核 /巨噬样细胞方向分化 ,高浓度时诱导细胞凋亡。 3 T871 3诱导HL 6 0细胞分化的过程伴随着c myc、c myb癌基因表达的下调 ,诱导HL 6 0细胞凋亡的过程伴随着c myc、bcl 2癌基因表达的下调。 结论 T871 3可能通过调节癌基因的表达发挥其诱导HL 6 0细胞分化和凋亡的作用。

Objective To investigate the effect of tadpole extract(T871 3) on tumor cells and its mechanism. Methods We studied the effects of T871 3 on proliferation, differentiation and apoptosis of HL 60 cells by cytomorphological observation, cytochemistry and TUNEL method. We also examined gene expression during the induction of apoptosis and differentiation in tadpole extract treated HL 60 cells by in situ hybridization and intact cell mRNA dot blot techniques. Results 1 T871 3 was able to inhibite HL 60 cells proliferation. 2 T871 3 was able to induce HL 60 cells to differentiate along monocyte macrophage lineage at low concentration, and apoptosis at higher concentration. 3 The differentiation of HL 60 cells was accompanied by downregulations of c myc,c myb gene expression, The apoptosis of HL 60 cells was accompanied by downregulations of c\|myc bcl 2 gene expression, suggesting that these genes may be involved in the apoptosis and differentiation process. Conclusion Tadpole extract may have effects on HL 60 cells through changing the oncogene expression. [