颅脑损伤后急性期脑脊液中IL-6和TNF-α的测定及其临床意义

Measurement and clinical significance of TNF-α and IL-6 in cerebrospinal fluid during acute period of brain injury

目的 检测白细胞介素 - 6 (IL - 6 )和肿瘤坏死因子 (TNF -α)在颅脑损伤后急性期脑脊液中的变化 ,探讨其临床意义。方法 采用放免法及酶联免疫法分别测定颅脑损伤后急性期脑脊液中TNF -α和IL - 6的浓度 ,并行健康对照。结果 颅脑损伤后IL - 6及TNF -α脑脊液中浓度第 1天便增高 ,第 3天达到高峰 ,第 7天开始下降。结论 TNF -α和IL - 6参与了颅脑损伤的病理生理过程 ,动态观察提示IL - 6可能和颅脑损伤病情程度有关。

Objective To investigate the change of tumor necrosis factor(TNF)and interleukin-6(IL-6) in cerebrospinal fluid(CSF)of patients during acute period of brain injury,and to explore the clinical significance.Method TNF and IL-6 in CSF of patients were measured by the methods of radioimmunoassay and enzyme-linked immunosorbent assay(ELISA) at day 1,3,5,7,10 post-injury.Control group was observed simultaneously.Results The concentration of TNF was increased at day 1 till day 5,and IL-6 in CSF were significantly increased in acute brain injured patients in the day 1 post-injury.The highest levels were reached at day 3 post-injury and continuous at that levels till the day 5 post-injury.Conclusion TNF and IL-6 were involved in the pathological process of brain injury and might have relevant with the severe of injury.