海洋生境芽孢杆菌(Bacillus spp.)的培养条件及产生的胞外抗菌蛋白

Cultural conditions of marine Bacillus spp. and effect on produced antagonistic protein

测试了 4株海洋芽孢杆菌的培养条件对其产生抗菌蛋白的影响 ;提取的粗蛋白的性质以及对 6种病原指示菌的抑菌活性 ,结果表明 ,4株海洋芽孢杆菌在2 2 1 6E ,EMB ,CM培养液 ;1 5～ 35℃ ;NaCl浓度 0 %～ 5 % ;pH3～ 9的条件下均能生长 ,适宜生长温度 2 0～ 2 8℃ ;NaCl浓度 1 %～ 3% ;pH5～ 8.菌株分别培养 84～1 0 8h抑菌活性最强 .(NH4) 2 SO4沉淀抗菌粗蛋白的最适饱和度为 5 0 %～ 70 % .粗蛋白对病原菌尤其对病原真菌具有强烈的抑制作用 .粗蛋白性质稳定 ,对高温和蛋白酶不敏感 .

The antagonistic protein production ,which inhibits 6 species fungi and bacteria from marine Bacillus spp., and the cultural conditions such as temperature, fermentation peried ,NaCl concentration and pH of 4 strians marine bacteria are studied. The result of test shows that suittable factor for both bacteria growth and antimicrobial substance produce are 20～28 ℃ ,84～ 108 h , NaCl 1%～3%, pH5～8 .Concentration of ammonium sulfate between 50% and 70% is the most fitting saturation for antagonistic protein precipitability. The proteins are insensitive to heat and protein and have high inhibitory value to pathogenetic fungi.