摘要
测试了 4株海洋芽孢杆菌的培养条件对其产生抗菌蛋白的影响 ;提取的粗蛋白的性质以及对 6种病原指示菌的抑菌活性 ,结果表明 ,4株海洋芽孢杆菌在2 2 1 6E ,EMB ,CM培养液 ;1 5~ 35℃ ;NaCl浓度 0 %~ 5 % ;pH3~ 9的条件下均能生长 ,适宜生长温度 2 0~ 2 8℃ ;NaCl浓度 1 %~ 3% ;pH5~ 8.菌株分别培养 84~1 0 8h抑菌活性最强 .(NH4) 2 SO4沉淀抗菌粗蛋白的最适饱和度为 5 0 %~ 70 % .粗蛋白对病原菌尤其对病原真菌具有强烈的抑制作用 .粗蛋白性质稳定 ,对高温和蛋白酶不敏感 .
The antagonistic protein production ,which inhibits 6 species fungi and bacteria from marine Bacillus spp., and the cultural conditions such as temperature, fermentation peried ,NaCl concentration and pH of 4 strians marine bacteria are studied. The result of test shows that suittable factor for both bacteria growth and antimicrobial substance produce are 20~28 ℃ ,84~ 108 h , NaCl 1%~3%, pH5~8 .Concentration of ammonium sulfate between 50% and 70% is the most fitting saturation for antagonistic protein precipitability. The proteins are insensitive to heat and protein and have high inhibitory value to pathogenetic fungi.
出处
《海洋学报》
CAS
CSCD
北大核心
2001年第4期87-92,共6页
基金
国家海洋局重点实验室基金资助项目 (98- 0 0 1)
山东省科技深海重点资助项目 (6 0 116 46 0 4)