L-四氢巴马汀对压力超负荷大鼠心肌肥厚细胞外基质增生的影响

Effects of L-tetrahydropalmatine on extracellular matrix proliferation of rats in hypertrophic myocardium caused by pressure overload

目的 观察L 四氢巴马汀 (L THP)对压力超负荷大鼠心肌肥厚细胞外基质 (ECM )增生的影响。方法 采用Wistar大鼠腹主动脉缩窄法建立压力超负荷 (PO ,n =10 )心肌肥厚模型 ,VG染色测定胶原体积分数 (CVF)和血管周围胶原面积 (PVCA)以观察ECM增生的程度 ;HE染色测量心肌细胞横径 (TDM)。PO +L THP(LT :n =10 )组 ,自腹主动脉缩窄术前 3d至术后 12wk通过饮水给予L THP(大约 1mg·g-1·d-1) ;另以假手术 (SO ,n =10 )组作为对照。结果 PO组TDM比LT和SO组增大 (P <0 0 5 ) ;CVF和2 0 0 0 12 0 6收稿 ,2 0 0 1 0 4 0 1再修回1 华中科技大学同济医学院培干中心 ,武汉 43 0 0 3 02 华中科技大学心血管疾病研究所 ,武汉 43 0 0 2 2作者简介 :刘承云 ,男 ,3 7岁 ,博士 ,现从事老年心血管病研究 ;毛焕元 ,男 ,72岁 ,教授 ,博士生导师PVCA高于LT及SO组 (P <0 0 1) ,而LT组与SO组相比 ,CVF和PVCA差异均无显著性 (P >0 0 5 )。结论 PO可致心肌肥厚和心肌纤维化 ,L

AIM To study the effects of L tetrahydropalmatine (L THP) on extracellular matrix (ECM) proliferation in hypertrophic myocardium of rats caused by pressure overload. METHODS The model (PO, n =10) was established with banding of abdominal aorta (PO). Collagen volume fraction (CVF) and perivascular collagen area (PVCA) were measured with Van Gieson staining to observe the severity of ECM proliferation. Myocardium transverse diameter (TDM) was measured with HE staining to observe myocardium hypertrophy. The group of PO+ L THP (LT, n =10) was given L THP (approximately 1 mg·g -1 weight per day orally in Drinking) from 3 days before the surgery to 12 weeks after it. The group of sham operated (SO, n =10) was used as control. RESULTS TDM、 CVF and PVCA were markedly increased (TDM: P <0 05; CVF and PVCA: P <0 01)in PO group than those in LT group and in SO group. CONCLUSION Myocardial hypertrophy and myocardial ECM proliferation can be caused by PO, and L THP markedly reduces this kind of effect.