摘要
目的 研究雷帕霉素 (RAPA)对ConA、PHA、LPS诱导的小鼠脾细胞增殖反应 ,混合淋巴细胞反应 ,脾T淋巴细胞亚型及细胞因子IL 2、IFN γ、TNF α含量的影响。方法 MTT法测定淋巴细胞增殖实验、混合淋巴细胞反应 ,流式细胞法测定脾T淋巴细胞亚型 ,结晶紫染色法测定TNF α ,E LASI法测定IL 2 ,IFN γ。结果 RAPA可显著抑制ConA、PHA和LPS诱导的小鼠脾细胞增殖和混合淋巴细胞反应(MLR) ,对小鼠脾T淋巴细胞亚型无明显影响。RAPA还可明显抑制小鼠脾淋巴细胞分泌IL 2 ,IFN γ ,但对小鼠腹腔巨噬细胞分泌TNF α无效。结论 RAPA抑制小鼠免疫功能的作用机制与CsA不同。
AIM To study the effects of RAPA on lymphocyte proliferation induced by mitogen and in mixed lymphocyte reaction(MLR), and the effects on T subpopulation and the content of IL 2,IFN γ,TNF α in mice. METHODS Lymphocyte proliferation induced by mitogens and MLR were detected with MTT colometeric assay. T cell subsets were measured with fluorescence activated cell sorter (FACs). IL 2,IFN γ and TNF α were detected by ELISA and biological assay. RESULTS RAPA inhibited murine lymphocyte proliferation induced by Con A, PHA or LPS, and also inhibited lymphocyte proliferation stimulated in MLR. RAPA had no significant effect on murine spleenic T lymphocyte subpopulation. RAPA inhibited ConA induced production of IL 2 of IFN γ by murine splenocytes, but had not inhibited LPS induced TNF α production by murine peritoneal macrophage. CONCLUSION The immunosuppressive mechanism of RAPA is distinct from CsA.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2001年第3期266-268,共3页
Chinese Pharmacological Bulletin