6-苯硫基-2-乙基-3-羟基-4(1H)-吡啶酮的抗氧化作用

Anti-oxidation role of 6-phenylthio-2-ethyl-3- hydroxy-4(1H)-pyridinone

目的 以正常大鼠组织匀浆、肝亚细胞及给药小鼠肝匀浆、血清等为材料研究 6 苯硫基 2 乙基 3 羟基 4(1H) 吡啶酮 (HDP)的抗氧化作用。方法 组织匀浆或肝亚细胞悬液与 6 苯硫基 2 乙基 3 羟基 4(1H) 吡啶酮共浴 ,以VitC/FeSO4 激发脂质过氧化反应 ,以丙二醛 (MDA)产生、还原型谷胱甘肽耗竭、线粒体膨胀作为评价脂质过氧化程度指标。小鼠ipHDP每天一次 ,连续 2 0d后摘眼球取血 ,分离血清 ,测定MDA含量 ,取肝制成匀浆 ,测定MDA含量。结果 6 苯硫基 2 乙基 3 羟基 4(1H) 吡啶酮可抑制VitC/FeSO4 激发的MDA的产生 ;抑制肝组织匀浆还原型谷胱甘肽耗竭及线粒体膨胀 ;降低给药小鼠肝匀浆及血清中的脂质过氧化物水平。结论 6 苯硫基 2 乙基 3 羟基 4(1H) 吡啶酮具有抗氧化作用。

AIM To study anti oxidation of 6 phenylthio 2 ethyl 3 hydroxy 4(1H) pyridinone (HDP) in tissues homogenates, livers subcellular and in serums from mice. METHODS Samples of tissues homogenates or livers subcellular were prepared with HDP, then addition of VitC/FeSO 4 initiated lipid peroxidation. The generation of malondialdehyde, exhaustion of glutathione in livers homogenate, swelling of mitochondria and contention of malondialdehyde in the serums of administered mice were examined. Mice had been administered HDP (ip) one time a day for twenty days. Then serums and livers homogenates were prepared for MDAdetermination. RESULTS HDP could inhibit VitC/FeSO 4 initiated lipid peroxidation in homogenates of different tissue (cerebral, heart, lung, liver and kidney) and in livers subcellular suspensions (plasma membrane, mitochondrion, microsome) of rats. HDP had potency to prevent exhaustion of glutathione in livers homogenate and swelling of mitochondria which followed lipid peroxidation. HDP could decrease the content of malondialdehyde in the livers and the serums of administered mice. CONCLUTION HDP has preventive action against lipid peroxidation.