大鼠提睾肌神经损伤后血管内皮细胞的凋亡

Apoptosis of vascular endothelial cells following cremaster nerve injury of rats

目的 研究大鼠提睾肌神经损伤后血管内皮细胞的凋亡 .方法 以免疫组织化学 SP法 ,对大鼠提睾肌神经损伤后的血管内皮细胞 Bcl- 2 ,Bax和 p5 3表达进行研究 .结果 支配提睾肌的神经损伤后 ,Bcl- 2 ,Bax和 p5 3的表达均有增强 ,但 Bax和 p5 3的表达明显强于 Bcl- 2 (P<0 .0 1和 P<0 .0 5 ) .神经损伤 1wk后凋亡水平最高 (与 2 4h组比较 P<0 .0 1) .结论 大鼠提睾肌血管内皮细胞失去神经支配后可产生过度凋亡 。

AIM To study the apoptosis of vascular endothelial cell with injured cremaster nerve of the rat. METHODS The expression of Bcl 2, Bax and p53 in endothelial cells with injured cremaster nerve was investigated by means of immunohistochemical SP method. RESULTS The expression of Bcl 2, Bax and p53 in endothelial cell of injured cremaster merve increased obviously, and reached the hightest level I weak after operation ( P <0.01 vs 24 h group) But the number of positive cell of Bax and p53 was larger than that of Bcl 2 ( P <0.01 and P <0.05). CONCLUSION Cremaster Nerve injury could result in apoptosis of the endothelial cells and might play an important role in the dysfunction of microcirculation.