猪视网膜神经节细胞的培养及超微结构

Pig retinal ganglion cells in culture

目的 建立视网膜神经节细胞 (retinal ganglioncells,RGCs)的体外培养方法 ,并研究视网膜不同类型神经细胞之间的相互作用 ,为 RGCs的体外实验研究奠定基础 .方法 进行猪视网膜细胞混合体外培养和神经节细胞的纯化培养 ,观察神经节细胞生长状态及轴突生长情况 ,研究视网膜不同类型神经细胞之间的相互作用 .结果 视网膜细胞混合培养时 ,神经节细胞生长良好 ,纯化后的神经节细胞间连接减弱 ,细胞存活时间缩短 .

AIM To establish a culture method for retinal ganglion cells (RGCs) and to investigate reciprocity among inhomogeneity cells of retina in order to lay a foundation for the experimental research in vitro . METHODS Retinal cells were cultured in commixture and retinal ganglion cells in purification, to observe the state of ganglion cells and its axon. RESULTS Retinal ganglion cells that cultured in commixture grew very well. The connects of purified ganglion cells were weakened, and the time of cells alive shortened. CONCLUSION Culturing retinal cells in commixture benefits the paste and growth of ganglion cells.