HBsAg阳性孕妇血清中乙肝病毒前S/S基因的相似株现象

Quasispecies of HBV preS/S gene in HBsAg carrying pregnant women

目的 探讨 HBs Ag阳性孕妇体内 HBV病毒前 S/ S基因变异的状况 .方法 应用全长 PCR技术从 4例表面抗原阳性孕妇血清中扩增出 HBV全长基因 ,将其克隆于 p UC19载体 ,每例随机筛选 5个阳性克隆 ,对其前 S/ S区进行序列分析 .结果 4例患者各克隆间均存在碱基的差异 ,此类患者体内 HBV株同源性较差 .结论 HBs Ag阳性孕妇体内 HBV株呈“相似株”

AIM To study the mutation of HBV preS/S gene in HBsAg carrying pregnant women. METHODS Full length polymerase chain reaction (PCR) was used to amplify whole HBV gene from sera of 4 HBsAg carrying pregnant women. The full length PCR products were cloned into pUC19 vector,5 clones were obtained every woman and subgenomic PCR was used to amplify HBV preS/S gene for DNA sequencing. RESULTS Compared with the wild type HBV C, all clones exhibited base insertion and substitution. CONCLUSION Quasispecies are the major characteristics in these HBsAg carrying pregnant women.