Brij78表面修饰对硬脂酸固态脂质纳米粒体内组织分布的影响

Effect of the modification of stearic acid solid lipid nanoparticles by chemical coating with Brij 78 on their body distribution in mice

目的 :研究Brij 78对硬脂酸纳米粒表面修饰后对硬脂酸纳米粒体内组织分布的影响。 方法 :采用“乳化蒸发 低温固化”的方法 ,分别用Brij 78和牛黄胆酸钠制备了硬脂酸纳米粒 ,以一级昆明小鼠为动物模型尾静脉注射纳米粒混悬液 ,于不同时间测定硬脂酸纳米粒在小鼠体内的组织分布情况。结果 :用Brij 78对硬脂酸纳米粒进行表面修饰后 (相对于牛黄胆酸钠组硬脂酸纳米粒 )对肾、心、肺和脾的靶向性提高了 84.8%、46 .7%、43.6 %和12 .5 %。用Brij 78对硬脂酸纳米粒进行表面修饰后其体内消除的相对速度由牛黄胆酸钠组的 6h提高到Brij 78组的 12h。结论 :用Brij78对硬脂酸纳米粒进行表面修饰后相对于牛黄胆酸钠能有效地增加纳米粒在非RES器官的分布。用Brij

To investigate the effect of surface modification of stearic acid solid lipid nanoparticles with Brij 78 on the body distribution. Methods: The stearic acid solid lipid nanoparticles were prepared by “emulsion evaperation solidfied at low temperature' method with two kinds of surfactants: Brij 78 and taurocholic acid sodium salt. Groups of five randomly selected Kun Ming mice of both sexes were used for each suspension and for each time point. After injection of each suspension into the tail vein, one group of mice were killed at each time point. Then the blood, liver, spleen, lung, heart, kidney, muscles and fat were removed and the dpm value of 100 μl blood and 50 mg of each tissue was determined. Results: Compared with the nanoparticles taurocholic acid sodium salt, the AUC (tissue)/AUC(blood) value of to spleen, lung, heart and kidney with the nanoparticles modified with Brij 78 was increased to 112.5%, 143.6%, 146.7% and 184.8% respectively. Conclusion: The distribution of stearic acid solid lipid nanoparticles into the non RES organs can be increased and the circulating time may be prolonged by modifying the nanoparticle surface with Brij 78.