p53蛋白与上颌窦鳞癌复发及预后的关系

The determination of p53 protein in maxillary sinus carcinoma and its prognosis value

目的 :探讨上颌窦鳞癌组织中 p5 3蛋白表达的诊断意义及其与复发及预后的关系。方法 :采用鼠单克隆抗体 DO- 7,免疫组化法检测 45例上颌窦鳞状细胞癌患者 (T2 N0 M0 )手术切除的癌组织中 p5 3蛋白含量 ,其过量表达提示 p5 3基因突变体存在。结果 :复发组中 88.2 %的癌组织中有 p5 3蛋白表达 ,显著高于对照组(2 8.6 % ) ,其差异有极显著性意义 (P <0 .0 1)。复发组中 6 1%的癌组织有较强的染色强度 ,高于对照组 (38% ) ,但其差异无显著性意义 (P >0 .0 5 )。结论 :p5 3蛋白可作为预测上颌窦鳞癌复发的基因水平的标记物 ,对其复发及预后具有重要意义。

Objective:To determine whether p53 protein overexpression correlated with early tumor recurrence in carcinoma of maxillary sinus.Method:Forty five patients with T 2N oM o squamous cell carcinoma of maxillary sinus were treated with operation and/or radiotherapy . The median follow up time was 3 years. The ratio of local control was 62.2 % (28 patients) , as control group ; The ratio of local recurrence and/or metastasis was 37.8 % (17 patients),as experiment group . Cancer specimens were obtained before treatment.p53 protein overexpression, which indicated a mutated p53 gene ,was analyzed by immunohistochemistry, using the mouse monoclonal antibody DO 7.Result:Approximately 88.2 % of the carcinoma that recurred locally overexpressed p53 protein , compared with only 28.6 % of those with local control (P< 0.01 ) .No significant relation was noted between p53 protein overexpression and pathohistologic grade .Intensity of staining did not predict tumor recurrece.Conclusion:The study demonstrated that the p53 protein is an important prognostic marker on gene level for maxillary sinus carcinoma.