抗原诱导免疫耐受的EAE大鼠中枢神经系统中一氧化氮合酶和细胞凋亡的变化

Changes of NOS and apoptosis in the CNS in EAE rats induced tolerance by feeding antigen

抗原髓鞘素于致敏注射前后给大鼠口服诱导免疫耐受 ,于致敏后第 14天 ,处死动物 ,检测中枢神经系统NADPH d和TUNEL阳性细胞 ,观察NOS表达和细胞凋亡的变化 ,结果为 1 EAE口服组nNOS的表达明显少于非口服组。 2 口服组与非口服组凋亡细胞数的变化趋势一致 ;相同的症状级别 ,凋亡细胞数也近似。提示CNS中 ,nNOS活性的降低可能与口服免疫耐受的形成有关 ,但CNS中的细胞凋亡与口服免疫耐受之间 ,未发现有明确的相关性。

In present study, the rats were fed with calf myelin before and after inoculation On 14 day post inoculation, NADPH d histochemical staining and in situ cell death detection were performed on CNS sections to observe the changes of NOS and apoptosis The results indicated: 1 The nNOS of the oral group was significantly reduced comparing to the non oral group of EAE 2 The oral group had the same changing curve with a single peak of apoptotic cells in number to the non oral during the course The amounts of apoptotic cells were similar in the same grade specimans from two groups It is indicated that the decrease of the activity of nNOS in rats' CNS may participate in the oral tolerance, but no evidence showed a definite correlation between apoptosis in CNS and oral tolerance