强直性脊柱炎并发虹睫炎易感性与LMP2和LMP7基因多态性的探讨

Relationship between the polymorphism of LMP2 and LMP7 genes and the susceptibility to iridocyclitis in patients with ankylosing spondylitis

目的 研究LMP基因多态性与强直性脊柱炎并发虹睫炎发病的关系。方法 应用PCR对正常人和病人进行HLA-B2 7检测以及LMP2和LMP7扩增。CfoI进行限制性酶切图谱分析。结果 强直性脊柱炎有虹睫炎 (AS +AAU)病史以及单纯虹睫炎 (AAU)患者LMP2基因BB纯合型较正常人以及强直性脊柱炎 (AS)患者明显增高 (P <0 .0 5 )。AS +AAU患者BB型OR =3.6 ,AAU患者BB型OR =5 .83。LMP7基因多态性无明显差别。结论 在我国汉人中 ,LMP2基因多态性与AS+AAU以及AAU发病存在明显相关关系。

Objective To evaluate the polymorphism of LMP2 and LMP7 genes in patients with ankylosing spondylitis(AS)and /or acute anterior uveitis (AAU).Methods HLA-B27 typing was performed and LMP2 and LMP7 amplified fragment length polymorphisms were determined after PCR and digestion with CfoI restriction enzyme.Results There was a significant increase of LMP2 BB genotypic frequency in patients with AS+AAU(85.7%)and AAU(87%)compared with controls (53.3%)and AS patients(62.5%)(P<0.05),but no difference in LMP7 genotype.Odds ratio (OR)relating LMP2 BB and AAU was 5.83 and OR for AS+AAU was 3.6.Conclusion The polymorphism of LMP2 gene is strongly associated with the incidence of AAU in AS patients and uncomplicated AAU patients. [WT5”HZ]