紫菜基因组DNA提取及酶切

Extraction and restriction digestion of genomic DNA from Porphyra

利用两种市售的DNA提取试剂盒对几种紫菜进行基因组DNA提取。该方法需要的组织量少 ,需时短 ,获得的基因组DNA纯度高 ,可被AFLP内切酶 ,特别是EcoRⅠ完全酶切 ,完全满足AFLP研究的需要。不同的DNA提取方法及提取出的基因组DNA的质量对酶切效果及以后的实验结果有影响。

High quality genomic DNA were extracted from Porphyra species with the DNA Isolation Kit for cell and Tissue (Roche). The results show this method is of special advantge to analyze the extraction from small sized samples. The genomic DNA extracted can be completely digested in AFLP research by the restriction endonucleases such as EcoR Ⅰ, Mse Ⅰ, TaqⅠ and Pst Ⅰ, et al, which is not the case reported that ‘Porphyra genomic DNA could not be restricted by EcoR Ⅰ’. The effects of model DNA quality on restriction digestion in AFLP and the problems in some other extracting methods prpviously reported are discussed also.