细胞融合法构建EPA和DHA高产异养藻株的研究

Study on the construction of high-EPA and DHA-containing heterotrophic microalga strain by cell fusion

利用细胞融合技术 ,将富含EPA和DHA的自养微藻绿色巴夫藻和生长迅速的异养微藻四鞭藻相融合 ,并筛选出兼养的融合藻株。融合藻株的总脂、EPA、DHA和EPA/DHA等各指标均比异养亲本四鞭藻有较大提高 ;在兼养条件下生长速率高于亲本微藻 ,脂肪酸组成以多不饱和脂肪酸为主 ;而在异养条件下以饱和脂肪酸和单不饱和脂肪酸为主。

The photoplasts of rapid growing heterotrophic microalgae Tetraselmis sp. and phototrophic microalgae Pavlova viridis with high level of EPA and DHA were fused by chemical cell fusion technique, and a fused strain of mixotrophic microalgae with high level of EPA and DHA and fast growing rate was selected. The fused strain has higher contents in total lipids, EPA, DHA and EPA/DHA than their parent heterotrophic Tetraselmis sp. Under mixotrophic conditions, the fused strain grows faster than its parents microalgae and the unsaturated lipid acids dominate the total lipid acids in it while under phototrophic conditions the saturated lipid acids dominate.