芦荟组培快繁技术研究

Studies on Tissue Culture and Micropro Pagation of Aloe

以MS为基本培养基 ,取芦荟幼嫩的茎、叶作为外植体 ,进行离体快繁研究。结果表明 ,芦荟最佳分化培养基的激素配比是 6-BA 3mg/L +NAA0 .2mg/L ;生根培养基NAA用量以 0 .3mg/L较好。在此优化条件下 ,芦荟的分化系数可达 6.3,再生苗的生根率可达 88.2 %。

The in vitro micropropagation of aloe was studied by using MS components as the basic medium and the young stem and leaf as explants.It was showed that the optimum hormone combinations were 6-BA 3mg/L+NAA 0.2mg/L for maximum shooting,and NAA 0.3mg/L for maximum rooting.Using these optimum hormone combination and concentration,the shooting frequency was 6.3 per explants,rooting frequency was up to 88.2% averagely.