烟草疫霉菌的培养及大量产生游动孢子囊和游动孢子方法的研究

Studies on the culture of Phytophthora nicotianae and the methods for producing zoosporangium and zoospore rapidly and enormously

烟草疫霉菌 (Phytophthoranicothianae)在燕麦培养基上生长良好 ,菌丝在滴加了土壤浸出液的皮氏培养液中 ,可在 3d内产生大量的游动孢子囊 ,游动孢子囊经低温处理后再培养 2

Phytophthora nicotianae could grow very well on oatmeal glucose agar medium.The hygha was cultured in 30 ml Petri solution which put 2-3 drops of filtered soil suspension . In this kind of blended solution, the hyphea of P. nicotianae could preduce a great quantity of zoosporangium by 3 days. Zoosporangium could produce a great quantity of zoospore after treated under low temperature and culturing 24 hours once more.