偶氮氯膦Ⅲ共振瑞利散射法测定蛋白质

Resonance Rayleigh Scattering Method for the Determination of Proteins with Chlorophosphonazo Ⅲ

研究了偶氮氯膦Ⅲ蛋白质体系的共振瑞利散射光谱 ,考察了体系的光谱特征 ,适宜的反应条件和主要影响因素 .蛋白质在一定的浓度范围内与散射强度呈线性关系 ,对于不同的蛋白质检出限在 8 2 4～ 10 4 0ng·mL-1之间 .考察了共存物质对测定的影响 ,方法具有良好的选择性 .此法用于人血清中蛋白质的测定 。

We investigated the spectra of resonance Rayleigh scattering (RRS) for chlorophosphonazo III-protein system, the spectral characteristics, the primary effect factors and optimum conditions of the reaction and established the quantitative relationships of the intensity of RRS to the concentration of protein. The calibration graph is linear over the range of 0～3 6 μg·mL -1 , and the detection limit of BSA is 18 5 ng·mL -1 . The method has fairly good selectivity with the investigation for the influence of some coexisting substances, and can be applied to the determination of protein in human serum and a pleasant result is obtained.