土曲霉CCTCCAF93044植酸酶基因的克隆及序列分析

Cloning and sequence analysis of a phytase gene from Aspergillus terreus

参考Hartingsveldt已发表的NRRL3135植酸酶 (phyA)基因序列 ,设计并合成了一对引物 ,应用PCR技术 ,以土曲霉CCTCCAF 930 44总DNA为模板 ,扩增出了不包括假定的信号肽序列的phyA基因 ,并将其克隆到PMD18-T载体上 ,测定了其核苷酸序列 ,并推导了其氨基酸序列 .该基因全长为 1433bp ,与已发表的NRRL3135的phyA基因的同源性为 97% ,编码一个含 45

Phytase catalyzes the hydrolysis of phytate(myo-inositol hexakisphosphate)to phosphate and myo-inositol, thereby improving the bioavailability of phytate phosphorus in plant foods and reducing phosphorus pollution of animal waste. A phytase gene from the A.terrus strain CCTCC 93044 was cloned by PCR and sequenced. The PCR primers were designed based on the published sequence of NRRL 3135 phyA. The phytase gene we cloned comprises 1 433 nucleotides without the putative signal sequence and encodes a polypeptide of 458 amino acids. The phytase gene shows 97% identity to the NRRL 3135 PhyA. It was planned to express this gene in yeast.