应用DOP-PCR与染色体涂染技术鉴别赤麂的Y染色体

Identification of Y Chromosome of Munticus Muntjac Vaginalis by Chromosome Painting and DOP-PCR

流式细胞仪分离小麂的Y染色体 ,并应用DOP PCR扩增后标记作为探针 ,分别与雄性及雌性赤麂中期核型进行染色体涂染 .结果表明 ,只有雄性赤麂Y2 染色体与探针有明显的杂交信号 ,说明只有Y2 染色体与赤麂的性别决定相关 ,排除了赤麂Y1染色体在性别决定中的作用 .

The Y chromosome of M reevesi were sorted by fluorescence activated chromosome sorting and were amplified and labeled by DOP-PCR.Painted probes of M reevesi were hybridized onto metaphase spreads obtained from female and male of M m vaginali.Strong signals were detected only on Y 2 chromosome.That indicates Y 2 is the real sex chromosome in M m vaginalis.