摘要
本试验以阔叶缬草 (ValerianafaurieiBriq .)为材料 ,就激素对叶外植体愈伤组织诱导和增殖的影响作了初步研究 .结果表明 ,培养基中添加 2 ,4-D能诱导愈伤组织产生 ,BA能促进愈伤组织增殖 .MS + 2 ,4-D 5μmol·L- 1 +BA 2 .5μmol·L- 1 为诱导叶块愈伤组织的最佳组合 .
In this experiment,leaf explant of Valeriana fauriei Briq. have been cultured in vitro for callus induce.Effects of hormones on callus induce and growth have been studied.2,4-D is necessary for callus induce.BA can stimulate callus growth.MS+2,4-D 5 μmol·L -1 +BA 2.5 μmol·L -1 is the best culture medium of callus induce.
基金
湖南省自然科学基金资助项目!( 98JJY2 0 65)
