一种制备过氧化物酶的新方法

A New Method of Producing Peroxidase

以meso-四(α,α,α,α-O-苯乙酰苯)卟啉为抗原,通过动物免疫、细胞融合等技术筛选细胞株3E4.该细胞株产生的抗体具有结合铁卟啉的能力,且表现出过氧化物酶的活力.该抗体对抗原和铁卟啉的亲和常数为3.65×10-9和8.20×10-8.抗体-铁卟啉复合物催化反应时,得到比活力为46.875U/mg,为天然HRP的18.99%.

Meso tetra(α,α,α,α Ο phenylacetyl benzene)porphyrin was used as complete antigen to elicit monoclonal antibody 3E4 through the immunization and cell fusion techniques. The antibody had the ability to combine with Fe porphyrin and showed the activity of peroxidase. The affinity constants for complete antigen and Fe porphyrin were 3.65×10 -9 and 8.20×10 -8 respectively.The activity of antibody Fe porphyrin complex with catalyzed reaction was 46.875?U/mg,which compared with natural HRP was 18.99%.