胃蛋白酶水解大豆蛋白的研究

Study of the hydrolysis of the soybean protein by pepsin

以大豆分离蛋白为底物，通过单因素分析、正交试验以及水解蛋白曲线的分析，确定了胃蛋白酶水解大豆蛋白的最佳水解条件；蛋白酶水解大豆蛋白的最佳水解条件： pH值为 2.0，水解温度为 37℃，酶与底物比为 7.0％ (质量百分比 )，底物质量浓度为 0.04 kg/L，反应时间为 24 h。

Using the soybean protein as the substrate,the best conditions of the hydrolysis of soybean protein have been found by making mono－ factor analysis,orthogonal test making out the curve of hydrolyzing the protein.In the experiments,optimum hydrolyzing conditions were pH 2.0,temperature 37℃ ,enzyme/substrate 7.0％ (W/W),sabstrate concen 4％ (W/V) and duration 24 h.