酵母发酵法分离纯化低聚异麦芽糖的研究

Study on Purification of Isomaltooligosaccharide by Yeast Fermentation

酿酒酵母As 2.109经驯化后，可用于发酵法分离纯化低聚异麦芽糖。优化后酵母发酵的最佳条件为：pH4.0，温度30～32℃，低聚异麦芽糖浓度250g/L，酵母膏浓度0.6g/L，CO(NH_2)_20.6g/L，Fe_2(SO_4)_30.08g/L，MgSO_41.2g/L。在此条件下，低聚异麦芽糖经38h发酵后，低聚异麦芽糖纯度由53.8％提高到90.3％，而葡萄糖被完全除去。

After taming,Saccharomyces cerevisiae(As2.109)could be used to purify isomaltooligosacchride by its selective fermentation.The optimum conditions for isomaltooligosacchride's purification were:pH 4.0,temperature 30～32℃,250g/L isomaltooligosacchride,0.6g/L yeast extracts,0.6g/L CO(NH2)2,0.08g/L Fe2(SO4)3，1.2g/L MgSO4.Under the optimum circumstance,after 38 hours' fermentation,the content of isomaltooligosacchride was purified up to 90.3％from previous 53.8％,while the glucose was completely eliminated.