两种紫杉烷类二萜化合物的分离鉴定

Isolation and Identification of Two Taxane Diterpenoid Compounds

目的 :从中国红豆杉细胞培养物提取紫杉烷类二萜化合物。方法 :95 %乙醇提取 ,溶剂萃取 ,硅胶柱层析 ,重结晶。结果和结论 :首次从中国红豆杉细胞培养物中分离得到两个紫杉烷类二萜化合物 ,经鉴定为14β (2 甲基丁酰氧 ) 2α ,5α ,10 β 三乙酰氧 紫杉 4(2 0 ) ,11 二烯 (1)和 5α 肉桂酰氧 7β ,9α ,10 β ,13α 四乙酰氧 紫杉 4(2 0 ) ,11 二烯 (2 )

AIM Taxane diterpenoid compounds are expected to be isolated from Taxus chinensis cell cultures. METHODS Cell cultures were extracted with 95% alcohol, distributed by different solvents, isolated via column chromatography on silica gel and purified by crystallization. RESULTS AND CONCLUSION Two taxane diterpenoid compounds were isolated from Taxus chinensis cell cultures for the first time.Their structures were identified as 14β (2 methylbutyryl)oxy 2α,5α, 10β triacetoxytaxa 4(20),11 diene(1) and 5α (cinnamoyl)oxy 7β,9α,10β,13α tetraacetoxytaxa 4(20),11 diene(2) respectively.