非水系统毛细管电泳分离麻黄碱类差向异构体

Separation of Ephedrine Isomers with Non-aqueous Capillary Electrophoresis

目的 :讨论了非水介质的组成等因素对麻黄碱类异构体分离的影响 ,并建立了最优的分离分析方法。方法 :采用 5 0mmol/L醋酸铵的甲醇溶液为介质 ,未加入任何其他添加剂 ,利用简单的非水毛细管区带电泳分离 5种麻黄碱类化合物。结果 :各麻黄碱类异构体在 8min内均达基线分离。结论 :毛细管电泳 (CE)分离弱酸、碱类药物时 ,采用非水溶液作背景电解质 ,不但可以增强待测物质的酸碱性 。

AIM\ Here the development of a simple, rapid and simultaneous method for the separation of five ephedrine alkaloids obtained with non aqueous capillary electrophoresis (NACE) were reported. The effects of the electrolyte, non aqueous solvent on the separation were also discussed. METHODS\ A running buffer of 50 mmol/L ammonium acetate in methanol was found to be the most suitable for this separation. RESULT\ The best separation was achieved within 8 minutes. CONCLUSION\ The separation is not only dependent on differences in the pK value of the alkaloids but also on the intramolecular interaction and solvation. So much improved selectivity could be achieved in nonaqueous medium.