摘要
目的 :探讨侧柏叶醇提取物的抗炎机理。方法 :以大鼠胸腔白细胞及兔血小板为材料 ,用高效液相色谱法测定细胞白三烯、12 羟十七碳三烯酸及 12 羟廿碳四烯酸的生成。结果 :侧柏叶醇提取物对白细胞内白三烯B4 及 5 羟廿碳四烯酸的生物合成有较强的抑制作用 ,其IC50 值分别为含生药 0 .4mg/ml及生药 0 .41mg/ml。侧柏叶醇提取物还可显著抑制血小板 12 羟十七碳三烯酸的生物合成。结论 :侧柏叶醇提取物中含有较强的抗炎成分 。
AIM The purpose is to investigate the Anti inflammatory mechanism of \%Biota orientalis\%. METHODS Using rat leukocytes and rabbit platelets as experimental material the biosynthesis of leukotriene, 12 hydroxyheptadecatrienoic acid(12 HHT) and 12 hydroxyeicosatetraenoic acid(12 HETE) in the cells was determined with HPLC. RESULTS \%Biota orientalis\% was shown to inhibit the biosynthesis of leukotriene B 4(LTB 4) and 5 hydroxyeicosatetraenoic acid(5 HETE) with IC 50 of 0.40 and 0.41 mg/ml crude herb, respectively. It was also displayed to inhibit biosynthesis of 12 HHT in the platelets. CONCLUSION \%Biota orientalis\% leaves contain anti inflammatory components and the anti inflammatory mechanism was related with inhibiting metabolism of arachidonic acid.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2001年第3期224-226,共3页
Journal of China Pharmaceutical University
基金
广东省卫生厅基金资助项目 ( 2 0 0 0 486 )~~