神经再生素对背根神经节细胞GAP-43和NF-L基因表达的影响

Effect of Nerve Regeneration Factor on GAP-43 and NF-L Gene Expression in Dorsal Roots Ganglia Cell

目的 :观察中药有效组分神经再生素作用于神经细胞生长过程中 ,相关基因的表达变化 ,探讨神经再生素促神经生长的分子生物学机制。方法 :采用RT PCR法 ,观察神经再生素在促大鼠背根神经节生长过程中(4h、12h、2 4h) ,生长相关蛋白 43(GAP 43)和神经丝蛋白 (NF L)基因表达的变化。结果 :神经再生素可使背根神经节细胞GAP 43和NF LmRNA的表达量增加。结论 :神经再生素可作用于体外培养的神经细胞 。

AIM To Detect the varying of gene expression in cultured dorsal root ganglion cells treated by Nerve Regeneration Factor and explore its molecular mechanism promoting to nerve growth. METHODS By RT PCR, the change of gene expression in GAP 43 and NF L on the cultured DRG cells with Nerve Regeneration Factor were studied during 4 h, 12 h, 24 h. RESULTS The expression of GAP 43 and NF L were increased by Nerve Regeneration Factor on DRG′s culture. CONCLUSION This study indicated Nerve Regeneration Factor may up regulate the gene expression associated nerve growth on cultured nervous cells.