摘要
目的:探讨胰岛素样生长因子I(IGF-I)对急性肾功能衰竭(ARF)的治疗作用。方法:建立大鼠缺血性急性肾衰竭模型,Wistar大鼠48只随机分成三组:假手术对照组(n=16),IARF组32只夹闭双肾动脉45min建立IARF模型(n=32),其中随机分16只为IGF-I组:每日皮下注射rhIGF-I一次(250ng.kg-1)。观察内生肌酐清除率(Ccr),24h尿蛋白,β2-MG,血、尿 IGF-I,血栓素B2(TXB2),6-酮-前列腺素 F1(6-keto-PCF1α)水平及肾脏组织形态学改变。结果:缺血后IARF组Ccr显著下降,24h尿蛋白,β2-MG下降,血、尿TXB2及肾重/体重增高,6-Keto-PGF1及尿 IGF-1水平降低,与对照组相比差异显著。 IGF-I组 Ccr升高,且较快恢复到对照水平;24 h尿蛋白,β2-MG及血、尿TXB2下降,6-keto-PGF1及尿IGF-I水平增高;肾组织学观察肾小管损伤程度减轻,肾小管上皮再生恢复快。结论:IGF-I能改善 IARF肾功能,减轻肾小管损伤程度,促进肾小管上皮再生恢复。
OBJECTIVE: To explore the mechanism of in- sulin-like growth factor-I(IGF-1) in acute renal failure(ARF). METHOD: Wistar rats were divided into three groups:Pseuoop- erative group (control, n = 16); IARF group(n = 32): rats were subjected to 45 minutes of bilateral renal ischemia; IGF-1 group(n = 16 ): a subcutaneous injection of rhIGF-I (250ng· Kg-1) was given per day after releasing clamped bilateral renal artery, Creatinine clearance(Ccr), urine protein Per 24 hours, serum and urine β2-microglobulin(β2-MG), IGF-1, thrombox- ane(TXB2), prostaglandin F1alpha (PGF1) were measured and rend histology was observed. Ccr concentration in IARF group decreased obviously, while proteinuria per 24 hoars, β2-MG, kidney weight/body weight, serum and urine levels of TXB2 were higher and serum and urine levels of PGF1α and urine IGF were lower than those in the control group. RESULTS: To compared with IARF group, Ccr concentration showed increase and a more rapid return toward hasal values, the serum and urine levels of TXB2, urine protein per 24 hours, β2-Mg reduced and serum and urine levels of PGF1α and urine IGF increased in IFG-I group. Kidneys from rats that had received IGF-I were improved in histopathological apperance. CONCLUSION: IGF-I improves renal function, hastens recovery and accelearates re- generation of damaged tubular epithelia following ischernic acute renal failure.
出处
《华西药学杂志》
CAS
CSCD
2001年第3期171-174,共4页
West China Journal of Pharmaceutical Sciences
基金
四川省卫生厅科研基金资助(962007)
四川省科委科研基金资助