RP-HPLC测定白花前胡中Pd-Ⅰa和Pd-Ⅱ的含量

DETERMINATION OF Pd-Ⅰa AND Pd-ⅡIN PEUCEDANUM PRAERUPTORUM BY RP-HPLC

目的：建立ＲＰ－ＨＰＬＣ测定白花前胡根中白花前胡丙素（Ｐｄ－Ｉａ）和白花前胡丁素（Ｐｄ－Ⅱ）的含量。方法：采用Ｓｈｉｍ－Ｐａｃｋ ＣＬＣ－ＯＤＳ柱，甲醇－水（７９：２１）为流动相，流速为 １．０ｍｌ·ｍｉｎ－１，以 Ｐｄ－Ⅰａ、Ｐｄ－Ⅱ为对照品，外标法峰面积定量，紫外检测（λ＝３２３ｎｍ）。结果：Ｐｄ－Ⅰａ、Ｐｄ－Ⅱ线性范围分别为０．０８～０．８μｇ和０．０６４～０．２８μｇ，ｒ＝０．９９９９和ｒ＝０．９９９９，平均回收率分别为 ９８．９０％和 ９８．９１％。结论：方法准确，简便，灵敏度高，快速，重复性好。

OBJECTIVE: To establish HPLC method for determining the contents of Pd-Ⅰa and Pd-Ⅱ in root of Peucedanum Praeruptorum. METHODS: The powdered samples was extracted with CHCl3 and CH3OH, the filtrate was analyzed by RP-HPLC, using ODS column, UV detection at 323 non, CH3OH-H2O(79:21 ) as mobile phase and Pd-Ⅰa, Pd-Ⅱ reference standard as external standard. RESULTS: The calibration curves showed good linearity over the range of 0.08 - 0.8 μg and 0.064 - 0.28 μg, r = 0.9999 and 0. 9999, the average mrecoveries were 98. 90% and 98. 91%, respectively. CONCLUSION: The method was simple, sensitive and reproducible, and can be used for the quality control of Chinese medicine Qianhu.