鼓槌石斛组织培养研究

Studies on Tissue Culture of Dendrobium chrysotoxum Lindl in Vitro

目的 :建立鼓槌石斛快速繁殖体系。方法 :组织培养 ,虫荧光素 荧光素酶生物发光法及苯酚 硫酸法。结果 :胚在光照及黑暗条件下均可以萌发 ,但苗的生长需要光照条件 ,MS ,1/2MS ,B5,N6培养基对苗的生长均比较适宜 ,0 .5mg·L-1NAA与 1mg·L-16 BA利于鼓槌石斛组培幼苗的生长与分化。三磷酸腺苷 (adenosinetriphosphate ,ATP)在鼓槌石斛培养时发生节律性变化。组培苗多糖含量 7.2 5 4% ,原植物为 2 .833%。结论 :光照对胚的萌发没有影响 ,分化出幼苗后 ,培养基中的激素、氮源及有机元素对苗的生长及分化有较大的影响 ,因此要及时将苗转移到附加NAA与 6 BA的MS ,1/2MS ,B5,N6培养基中。ATP可作为鼓槌石斛培养时的动态指标 ,反应其发育过程中对营养和能量的需求。

Objective:To set up a system for the culture of Dendrobium chrysotoxum in vitro. Method:Tissue culture,fire fly luminescence and phenol H 2SO 4 method. Result:The embryo could germinate with or without light, the MS, 1/2MS, B 5, N 6 mediums are suitable to the growth and the differentiation of sprout with light, 0.5 mg·L -1 NAA and 1 mg·L -1 6 BA, and ATP have regular changes, the content of polysaccharide was 2.833% in plant and 7.254% in sprout. Conclusion:The light has no effects on the embryo germination, but the phytohormone,nitrogen source and organized elements are important to the growth and differentiation of the sprout which should be transferred to the MS, 1/2MS, B 5, N 6 mediums in time supplemented with NAA与6 BA, ATP may be served as the dynamic indication of nourishment demand in the plant. The content of polysaccharide in the sprout is higher and can be utilized.